Fig. 1

Schematic representation of Citrus leaf blotch virus (CLBV)-based VIGS vector construction. Schematic representation of full-length infectious cDNA clones of CLBV with its open reading frames (ORF) placed between enhanced 35S promoter of cauliflower mosaic virus (CaMV) at the 5′ end, and nopaline synthase terminator (Nos ter) at the 3′ end in the ternary shuttle vector pCY. Introducing a unique restriction sites SmaI after coat protein (CP) gene. 471 bp fragment of green fluorescent protein (471 gfp) was cloned using unique restriction sites SmaI to generate pCLBV201-gfp471, similarly, 391 bp fragment of phytoene desaturase (391 pds), 245 bp fragment of sulfur (245 su), 376 bp fragment of LATERAL ORGAN BOUNDARIES 1 (376 lob) genes were cloned to generate pCLBV201-pds391, pCLBV201-su245, and pCLBV201-lob376, respectively. Mtr, methyltransferase motif; AlkB, AlkB-like peptidase; P-Pro, protease; Hel, helicase-like domain; RdRP, RNA-dependent RNA polymerase domain; MP, movement protein