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Fig. 2 | Phytopathology Research

Fig. 2

From: A simple and effective VIGS system facilitates the control of citrus canker by silencing CsLOB1

Fig. 2

Silencing phenotypes of gfp in N. benthamiana transgenic line 16 c by Agrobacterium-mediated injection-infiltration. a The seven days post inoculation (dpi), b 20 dpi, c 30 dpi, d 40 dpi, e silencing phenotypes in leaves. f The silencing phenotypes in flowers. The relative expression level of the gfp gene of N. benthamiana transgenic line 16 c inoculated with pCLBV201-gfp471 or pCLBV201-wt by Agrobacterium-mediated injection-infiltration. 7 d, 7 dpi; 20 d, 20 dpi; 30 d, 30 dpi; 40 d, 40 dpi. The amount of gfp transcript was normalized to that of the actin gene in the same plants, the average expression in control plants infected with pCLBV201-wt was considered 1.0, and the normalized expression in plants infected with pCLBV201-gfp471 was relative to the average of the control plants. Bars represent standard deviation values and statistical significance was determined using student’s t-test (*P < 0.05, **P < 0.01)

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