Fig. 3

Silencing phenotypes of su and pds in Eureka lemon by Agrobacterium-mediated vacuum-infiltration (AVI). a Eureka lemon plants inoculated with pCLBV201-su245 at 30 dpi. c Eureka lemon plants inoculated with pCLBV201-pds391 at 30 dpi. b and d Eureka lemon plants inoculated with pCLBV201-wt at 30 dpi. e and f The relative expression levels of the su and pds genes of Eureka lemon inoculated with pCLBV201-su245 and pCLBV201-pds391 or pCLBV201-wt at 30 dpi, respectively. For each gene, the amount of mRNA was normalized to that of the actin gene in the same plants. The average expression in control plants infected with pCLBV201-wt was considered 1.0, and the normalized expression in plants infected with the pCLBV201-su245 and pCLBV201-pds391 was relative to the average of the control plants. Bars represent standard deviation values and statistical significance was determined using student’s t-test (*P < 0.05). g and h The contents of total chlorophylls and leaf carotenoid of Eureka lemon inoculated with pCLBV201-su245 and pCLBV201-pds391 or pCLBV201-wt at 30 dpi, respectively