Fig. 5
From: A simple and effective VIGS system facilitates the control of citrus canker by silencing CsLOB1
Silencing phenotypes of su in Eureka lemon by Agrobacterium-mediated vacuum-infiltration (AVI) after grafted onto Ziyang xiangcheng (Citrus junos Sieb. ex TaKaRa) rootstock four years. a and b Eureka lemon plants inoculated with pCLBV201-su245. c Eureka lemon plants inoculated with pCLBV201-wt. d The relative expression levels of the su gene of Eureka lemon inoculated with pCLBV201-su245 or pCLBV201-wt after four years. The amount of mRNA was normalized to that of the actin gene in the same plants. The average expression in control plants infected with pCLBV201-wt was considered 1.0, and the normalized expression in plants infected with the pCLBV201-su245 was relative to the average of the control plants. Bars represent standard deviation values. e RT-PCR detection of inserted fragment of Eureka lemon. 1–3, pCLBV201-su245 inoculated plants; 4–6, pCLBV201-wt inoculated plants. M, DL2000 marker. Bars represent standard deviation values, statistical significance was determined using student’s t-test (*P < 0.05, **P < 0.01)