Table 1 List of primers used in this study
From: A simple and effective VIGS system facilitates the control of citrus canker by silencing CsLOB1
Primer name | Sequence (5′–3′) | Application |
|---|---|---|
CP1125-F | tataacgtgtgactagtgtaaatagttagtACTTATCTAAATTAATCTAGATAGTGTACATCCA | Amplification of fragment CP1125 |
CP1125-R | CTACATTTCTAAGAGTTTTGCTTTGTT | |
CP1080-F | aaaaacaaagcaaaactcttagaaatgtagcccgggTCCCGAATTCTGGCATG | Amplification of fragment CP1080 |
CP1080-R | actatagcagcggaggggttggatcaaagtACTTTGATCCCGAGGGGAACCCTG | |
GFP471-F | tgccagaattcgggacccTCACTGGAGTTGTCCCAATT | Construction of pCLBV201-471gfp |
GFP471-R | ctcttagaaatgtagcccAACTTGTGGCCGAGGATGTT | |
SU245-F | tgccagaattcgggacccCGGGCTCTGTTTGCAGTTACC | Construction of pCLBV201-245su |
SU245-R | ctcttagaaatgtagcccCGGGGAGTAAATCTACCAAGGACC | |
PDS391-F | tgccagaattcgggacccAGCCTTTGCTTCAGCGTTTCTGAAAGTGCTTTC | Construction of pCLBV201-391pds |
PDS391-R | ctcttagaaatgtagcccGTCTCATACCAGTTCCCGTCCCCATCTTTCC | |
LOB376F | tgccagaattcgggaccc GAATGCAAACACAAAATTAAT | Construction of pCLBV201-376lob |
LOB376R | ctcttagaaatgtagcccAACTTGT GGCCGAGGATGTT | |
CLBV-F | CAATGCAGCAATTCCAACTGA | RT-PCR detection of CLBV infection |
CLBV-R | GCGGTATCCCCTTTAGCATC | |
Qgfp-F | AAAGCCAACTTCAAGACCC | RT-PCR detection of GFP mRNA |
Qgfp-F | AAAGGGCAGATTGTGTGG | |
Qsu-F | GGAGGAGAGAGCTCGATTTG | RT-PCR detection of SU mRNA |
Qsu-R | GAGAGAACTCCTGGCTGAGG | |
Qpds-F | TGTATTTGCCACACCTGTTG | RT-PCR detection of PDS mRNA |
Qpds-R | TGGATGTTGATGACTGGAAC | |
Qlob-F | AAAGCCAACTTCAAGACCC | RT-PCR detection of LOB mRNA |
Qlob-F | AAAGGGCAGATTGTGTGG | |
Xac F07 | GAGTCGCCTACCGAGAAATCC | RT-PCR detection of xac mRNA |
Xac R07 | ACCACGGCAGGGTGAAGAC | |
CitActin-F | CATCCCTCAGCACCTTCC | RT-PCR detection of citrus Actin mRNA |
CitActin-R | CCAACCTTAGCACTTCTCC | |
Tactin-F | AAGGGATGCGAGGATGGA | RT-PCR detection of tobacco Actin mRNA |
Tactin-R | CAAGGAAATCACCGCTTTGG |