Fig. 2
Comparison of lignin contents between smut-resistant and smut-susceptible sugarcane varieties. a Observation of lignin in buds of GT42, FN41, ROC22, ZZ1, ZZ6, and ZZ9 after inoculation with S. scitamineum at 0, 24, 48, and 72 h. The lignin is stained with red color by phloroglucinol (cross-section of the bud). Black arrows indicate lignin and green arrows indicate trichomes. Scale bars = 50 μm. b Quantification of lignin content in buds of different varieties. Six biological replicates were included in each sample. SPSS Software was used to analyze the lignin content of each sugarcane at different time points, and the significance is denoted above each column. Values in the same column with different letters were significantly different (p < 0.05), calculated using one-way analysis of variance (ANOVA) followed by multiple Duncan tests