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Fig. 4 | Phytopathology Research

Fig. 4

From: Four sugarcane ScDIR genes contribute to lignin biosynthesis and disease resistance to Sporisorium scitamineum

Fig. 4

Recombinant ScDIR proteins mediate the synthesis of the lignin precursor pinoresinol. a SDS‒PAGE analysis of ScDIR proteins. Molecular mass marker (lane 1), uninduced total proteins (lane 2), IPTG-induced total proteins (lane 3), supernatant (lane 4), precipitate (lane 5), miscellaneous protein (lanes 6 and 7), and purified protein (lane 8). GST-ScDIR5 is a recombinant ScDIR5 with a GST tag. b HPLC analysis of the synthesis of pinoresinol from coniferyl alcohol in the presence of recombinant ScDIR proteins. Mobile phase A is 10% formic acid + 0.1% TFA and mobile phase B is 15% methanol/acetonitrile (40:60; v/v). Control group contains coniferol alcohol standard and pinoresinol standard, and experimental group contains coniferol + GST, coniferol + GST-ScDIR5, coniferol + GST-ScDIR7, coniferol + GST-ScDIR11, and coniferol + GST-ScDIR40. Three biological replicates were performed for each sample

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Phytopathology Research

ISSN: 2524-4167