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Fig. 7 | Phytopathology Research

Fig. 7

From: Four sugarcane ScDIR genes contribute to lignin biosynthesis and disease resistance to Sporisorium scitamineum

Fig. 7

Overexpression of ScDIR genes in sugarcane enhances resistance to S. scitamineum. a The progression of whip development in ScDIR transgenic lines. Tissue culture-derived plantlets of the ScDIR transgenic lines were inoculated with two mating strains of S. scitamineum JG35 and JG36, by root dipping method. “n” represents the number of sugarcane-inoculated lines. b Symptoms of plantlets inoculated with S. scitamineum. Arrows indicate black whips. Scale bars = 20 cm. c Histopathology was carried out in the plantlets by dissecting and staining them with trypan blue. Arrows indicate fungal hyphae in vivo. Scale bars = 25 µm. d Quantification of S. scitamineum bE gene copies in ScDIR transgenic lines at 150 days post-inoculation. Three biological replications were conducted. Values in the same column with different letters indicate significant difference from the control (p < 0.01) using one-way analysis of variance (ANOVA) followed by multiple Duncan tests

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Phytopathology Research

ISSN: 2524-4167