Fig. 8
Overexpression of the ScDIR5, ScDIR7, ScDIR11, and ScDIR40 genes increased the lignin content in sugarcane. a Observation of lignin in wild-type sugarcane (WT), sugarcane with empty vector (35S-transgenic), and transgenic sugarcane (ScDIR5-transgenic, ScDIR7-transgenic, ScDIR11-transgenic, and ScDIR40-transgenic lines) at 150 days post-inoculation using phloroglucinol staining. Arrows indicate lignin. Scale bars = 25 µm. b Quantitative analysis of lignin content of different samples. Six biological replicates were included in each sample. SPSS software was used to analyze the lignin content of each sugarcane at different time points, and the significance is denoted above each column. Values in the same column with different letters indicate significant difference (p < 0.05) using one-way analysis of variance (ANOVA) followed by multiple Duncan tests