Fig. 2

MoGRP1 is indispensable for hyphal growth, stress responses, and conidiation. a Mycelial growth of the wild-type strain P131, the ΔMogrp1 mutant kg1, and one ΔMogrp1 complementation transformant cg1g on CM, OTA, MM, MM-C, and MM-N plates at 28 °C for 5 days. The black circle indicates the extent of mycelia grown on MM-N and MM-C plates; b Column diagram showing colony diameter of strains cultured on different media in (a). The mean ± SD was calculated based on three independent experiments measuring three plates in each replicate. An asterisk marks significant differences among strains according to a T-test (P < 0.05); c Conidiation structures of strains P131, kg1, and cg1g cultured on OTA plates. Bar, 30 μm; d Conidiation capacity of strains P131, kg1, and cg1g cultured on OTA plates. The mean ± SD was calculated based on three independent experiments measuring three plates in each replicate. *P < 0.05; e Conidial morphology of strains P131, kg1, and cg1g. Bar, 20 μm; f Percentage of conidia with 0-, 1-, and 2- septa of strains P131, kg1, and cg1g. The mean ± SD was calculated based on three independent experiments measuring at least 100 conidia in each replicate. *P < 0.05