Fig. 3
MoGRP1 is required for plant infection and infection-related morphogenesis. a Disease lesions formed on rice seedlings (left panel), barley seedlings (middle panel), and abraded rice leaves (right panel) by inoculation of the wild-type strain P131, the ΔMogrp1 mutant kg1, and one ΔMogrp1 complementation transformant cg1g. Typical disease lesions were photographed at 5–7 dpi; b Appressorium formation on glass coverslips (upper panel) and its percentages (lower panel) by strains P131, kg1, and cg1g at 12 hpi. The mean ± SD was calculated based on three independent experiments measuring at least 100 germinated conidia in each replicate. Bar, 20 μm. *P < 0.05; c Appressorium-mediated penetration and invasive hyphal development in epidermal cells of barley leaves by strains P131, kg1, and cg1g at 24, 36, and 48 hpi. Bar, 20 μm; d Column diagram showing the percentage of penetration pegs forming invasive hyphae in the epidermal cells of barley leaves by strains P131, kg1, and cg1g at 24 hpi. The mean ± SD was calculated based on three independent experiments measuring at least 100 appressoria in each replicate. *P < 0.05; e Column diagram showing the percentage of invasive hyphae extending into neighboring cells in the epidermis of barley leaves by strains P131, kg1, and cg1g at 48 hpi. The mean ± SD was calculated based on three independent experiments measuring at least 60 infection sites in each replicate. *P < 0.05