Fig. 7

Both the RRM and the first two RGG motifs are essential for the full function of MoGrp1. a Structure diagram of N-terminal 3 × Flag tagged MoGrp1 (cg1f), C-terminal GFP tagged MoGrp1 (cg1g) and their mutated alleles, including RNP-2 deletion (dRRM, MoGrp1^Δ119–125), N-terminal deletion (dN, MoGrp1^Δ1–74), glycine-rich domain complement transformant (dNR, MoGrp1^Δ1–173), deletion from the 3rd RGG motif to the end (Δ_265–324, MoGrp1^Δ265–324), deletion from the 2nd RGG motif to the end (Δ_191–324, MoGrp1^Δ191–324), deletion from the 1st RGG motif to the end (Δ_182–324, MoGrp1^Δ182–324), deletion from 182 to 190 aa (Δ_182–190, MoGrp1^Δ182–190), transformant containing RRM and 1st RGG (dNG, MoGrp1^75–190), and AGG mutant (AGG, MoGrp1^{R182A, R191A}); b Pathogenicity test of diverse domain-complemented strains in rice seedlings. Typical disease lesions were photographed at 5–7 dpi; c Colony diameter of diverse domain-complemented strains on OTA plates at 28 °C for 5 days. The mean ± SD was calculated based on three independent experiments measuring three plates in each replicate. *P < 0.05; d Conidiation capacity of diverse domain-complemented strains on OTA plates. The mean ± SD was calculated based on three independent experiments with measuring three plates in each replicate. *P < 0.05