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Table 6 Inoculation and incubation conditions and disease scoring scales used in the four tests of this study

From: The overlapping continuum of host range among strains in the Pseudomonas syringae complex

Test A-1998 B-2008 C-2013 D-2017
Date of inoculations
 Block 1 Feb-June 1998 Mar-June 2008 Mar-June 2013 May–June 2017
 Block 2 May-Sept 1998 July-Nov 2008   
# Plants/spp./strain/block 3 3 4 5
Total # plants tested/ strain/plant species 6 6 4 5
Age of plants Dicots: 2-leaf stage (2 trifoliate leaves for bean plants), except peas which were inoculated at the 4-leaf stage. Monocots: 2–3 leaf stage. Annual plants: one month after sowing (3 to 5 true leaves); 2-year-old Prunus spp.; 3-month-old hazelnut, kiwifruit, oleander and poplar plants All plants: one month after sowing (2–6 true leaves)
Inoculum concentration 5 × 107 to 1 × 108 CFU/mL 108 CFU/mL 108 CFU/mL
Inoculation of herbaceous plants Infiltration in leaf blade near the base of the leaf (ca. 50 μL) 10 droplets (10 μL) on a leaf and 1 droplet (10 μL) injected into stem on each plant. Infiltration in leaf blade near the base of the leaf (10 μL) after wounding the site with a sterile plastic stick.
Inoculation of woody plants    1 droplet (10 μL) on a scar at the petiole-stem junction, sealed with parafilm.  
Scoring method 0: apparent no reaction;
1: HR-like reaction that does not evolve;
2: symptoms on < half of leaf;
3: symptoms on > half of the leaf blade.
0: no apparent reaction;
1: < 5 lesions/leaf, no symptoms on stems;
2: 5–10 lesions/leaf, no symptoms on stems; 3: > 10 lesions/leaf, no symptoms on stems; 4: some dead leaves, no symptoms on stems;
5: leaf score 1 with lesions on stems or petioles < 5 mm long;
6: leaf score 2 with lesions on stem or petiole from 5 to 10 mm;
7: leaf score 3 with necrosis on stem or petiole > 10 mm;
8: dead plant
0: no apparent reaction;
1: weak symptoms, no necrosis;
2: necrosis at point of inoculation without spreading;
3:-necrosis up to 25% of leaf blade;
4: necrosis from 25 to 50% of leaf blade;
5: necrosis for > 50% of leaf blade;
6: wilting of entire leaf.
Scoring dates (days after inoculation) 2 and 7 days   Herbaceous plants: 5, 10 and 15 days. Woody plants: 15, 30, 45, 60 days 2 and 6 days
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