From: The overlapping continuum of host range among strains in the Pseudomonas syringae complex
Test | A-1998 | B-2008 | C-2013 | D-2017 |
---|---|---|---|---|
Date of inoculations | ||||
Block 1 | Feb-June 1998 | Mar-June 2008 | Mar-June 2013 | May–June 2017 |
Block 2 | May-Sept 1998 | July-Nov 2008 | ||
# Plants/spp./strain/block | 3 | 3 | 4 | 5 |
Total # plants tested/ strain/plant species | 6 | 6 | 4 | 5 |
Age of plants | Dicots: 2-leaf stage (2 trifoliate leaves for bean plants), except peas which were inoculated at the 4-leaf stage. Monocots: 2–3 leaf stage. | Annual plants: one month after sowing (3 to 5 true leaves); 2-year-old Prunus spp.; 3-month-old hazelnut, kiwifruit, oleander and poplar plants | All plants: one month after sowing (2–6 true leaves) | |
Inoculum concentration | 5 × 107 to 1 × 108 CFU/mL | 108 CFU/mL | 108 CFU/mL | |
Inoculation of herbaceous plants | Infiltration in leaf blade near the base of the leaf (ca. 50 μL) | 10 droplets (10 μL) on a leaf and 1 droplet (10 μL) injected into stem on each plant. | Infiltration in leaf blade near the base of the leaf (10 μL) after wounding the site with a sterile plastic stick. | |
Inoculation of woody plants | 1 droplet (10 μL) on a scar at the petiole-stem junction, sealed with parafilm. | |||
Scoring method | 0: apparent no reaction; 1: HR-like reaction that does not evolve; 2: symptoms on < half of leaf; 3: symptoms on > half of the leaf blade. | 0: no apparent reaction; 1: < 5 lesions/leaf, no symptoms on stems; 2: 5–10 lesions/leaf, no symptoms on stems; 3: > 10 lesions/leaf, no symptoms on stems; 4: some dead leaves, no symptoms on stems; 5: leaf score 1 with lesions on stems or petioles < 5 mm long; 6: leaf score 2 with lesions on stem or petiole from 5 to 10 mm; 7: leaf score 3 with necrosis on stem or petiole > 10 mm; 8: dead plant | 0: no apparent reaction; 1: weak symptoms, no necrosis; 2: necrosis at point of inoculation without spreading; 3:-necrosis up to 25% of leaf blade; 4: necrosis from 25 to 50% of leaf blade; 5: necrosis for > 50% of leaf blade; 6: wilting of entire leaf. | |
Scoring dates (days after inoculation) | 2 and 7 days | Herbaceous plants: 5, 10 and 15 days. Woody plants: 15, 30, 45, 60 days | 2 and 6 days |