Fig. 5

Validation of some DEGs by RT-qPCR. Reverse transcription quantitative PCR (RT-qPCR) analysis on the relative mRNA levels of the indicated four representative resistance type A genes (a), four representative resistance type B genes (b), four representative resistance type C genes (c), one representative resistance type C gene and one gene not in the classified group (d), two representative susceptibility type C genes (e), three representative susceptibility type A genes (f). Note in (a) that the mRNA levels were highly induced at and after 3 dpi in Col-0, but remained at low levels in pad4–1 sid2–1, and in (f) that the mRNA levels of CLE1 and At3g59930 were remarkably up-regulated at and after 6 dpi in pad4–1 sid2–1. Relative mRNA levels were normalized to 0 dpi of Col-0 that was arbitrarily set as 1. Values are means of three technique replications. Error bars indicate SD. Asterisks above the bars indicate significant differences between Col-0 and pad4–1 sid2–1 at P < 0.01 as determined by student’s t-test. All the experiments were repeated two times with similar results