Fig. 1
From: A Phytophthora capsici virulence effector associates with NPR1 and suppresses plant immune responses
RxLR48 interacts with NPR1. a Interaction between RxLR48 and NPR1 in yeast. RxLR48 was cloned into bait plasmid pGBKT7 (BD) and NPR1 was cloned into prey plasmid pGADT7 (AD), respectively. Combination of BD-53 and AD-T was used as a positive control while BD-Lam and AD-T as a negative control. Yeast transformants were grown on selective medium (SD) lacking Tryptophan (T) and Leucine (L) and selected on SD lacking Tryptophan (T), Leucine (L), Histidine (H) and Adenine (A). The plates were photographed at 3 days after inoculation. b Interaction between RxLR48 and NPR1 in Arabidopsis. RxLR48-FLAG and NPR1-HA were transiently co-expressed in Arabidopsis protoplasts. RxLR48 together with GFP was used as a negative control. Total proteins were extracted, followed by immunoprecipitation with an α-FLAG antibody (α-FLAG IP). Input and the bound proteins were detected via immunoblot using α-FLAG and α-HA antibodies, respectively