Fig. 5
From: A Phytophthora capsici virulence effector associates with NPR1 and suppresses plant immune responses
RxLR48 inhibits protein degradation of NPR1. a Stabilization of NPR1 by RxLR48 in vivo. RxLR48-FLAG and NPR1-HA were transiently co-expressed in Arabidopsis, and protein levels were analyzed by immunoblots with indicated antibodies. Protoplasts were treated with 0.25 mM SA before incubation. Numbers below the blots represent relative abundances of NPR1 and solo expression of NPR1 is set to 1 as the reference. Asterisks indicate significant differences (**, P < 0.01 compared with the reference, Dunnett’s test). Ponceau staining was used to show equal loading. b Increased accumulation of NPR1 by RxLR48 or MG132. Protoplasts were transiently transfected with the indicated constructs, followed by DMSO or MG132 (100 μM) treatment before incubation overnight. Protein levels were analyzed and relative abundances of NPR1 were calculated as described above. Asterisks indicate significant differences (**, P < 0.01 compared with the reference, Dunnett’s test)