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Fig. 3 | Phytopathology Research

Fig. 3

From: Development of polyclonal antiserum against movement protein from Potato leafroll virus and its application for the virus detection

Fig. 3

Sensitivity analysis of PLRV-MP antiserum by Western blotting. a, b Lane M: PageRuler Prestained Protein Ladder, and the rest of the lanes show multiple dilutions of stock protein from the PLRV-infected Nicotiana benthamiana leaves (10-, 20-, 40-, 80-, 160-, 320-, 640-, 1280-, and 2560-fold), with anti-MPPLRV at the ratio of 1:10000 and 1:20000 respectively. c Lane M: PageRuler Prestained Protein Ladder, and the rest of the lanes show different concentrations of purified MP fusion protein (20, 10, 5, 2, 1, 0.5, 0.25, 0.1, 0.05, 0.025, 0.0125, and 0.005‚ÄČng), with antiserum at the ratio of 1:20000

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