Diversity of Pythium spp. associated with soybean damping-off, and management implications by using foliar fungicides as seed treatments

Table 2 Damping-off incidence (%) of Pythium isolates on soybean variety Pioneer 22T61RR in pathogenicity tests conducted in greenhouse^x

Cups labeled	Isolate No.	Identity with GenBank (%)	GenBank Accession No.	Pythium spp.	Incidence (%)^y
1	D1–2	100% (814/814 bases)	AY598626	P. inflatum	2.4c
2	1–4	100% (870/870 bases)	KU210728	P. ultimum var. ultimum	58.3b
3	C1–2	99% (855/864 bases)	HQ643723	P. orthogonon	3.6c
4	-4	100% (826/826 bases)	AY598624	P. torulosum	2.4c
5	D2–1	100% (822/822 bases)	AY598626	P. inflatum	2.4c
6	A2–1	100% (823/823 bases)	AY598626	P. inflatum	1.2c
7	2–3	100% (876/876 bases)	KU210728	P. ultimum var. ultimum	79.8a
8	?	100% (662/662 bases) 100% (659/659 bases)	KU211001 KT429653	P. ultimum var. ultimum or P. ultimum var. sporangiferum	58.3b
9	??	100% (886/886 bases)	KU211001	P. ultimum var. ultimum or P. ultimum var. sporangiferum	51.2b
10	IA-Gr-21^z			P. irregulare	14.3c

^xResults are average of 12 replications with each replication planted with 7 seeds. ^yMeans within column followed by the same letter(s) are not significantly different from each other at 5% level of significance (P < 0.05). ^zIowa isolate from Dr. Alison Robertson, Iowa State University