Fig. 1

Design of LAMP primers for detection of the E198K/A/V mutations of TUB2 in Botrytis cinerea. a Nucleotide sequence alignment of sensitive and resistant genotypes. The sequences used as LAMP primers are indicated by bars. Point mutations are shown underlined with one line and mismatches are shown underlined with double lines. For each mutation, the F2 primer ends with its corresponding mutated nucleotide. b Schematic representation of the LAMP primers used in this work. The inner primer FIP consists of F1c and F2, and BIP comprised of B1c and B2. Primers ending with ‘c’ are complementary to their counterparts without ‘c’