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Fig. 2 | Phytopathology Research

Fig. 2

From: Rapid detection of benzimidazole resistance in Botrytis cinerea by loop-mediated isothermal amplification

Fig. 2

LAMP primer set designed to amplify the E198K, E198A and E198V mutations in Botrytis cinerea, respectively. a LAMP products detected with 1000 × SYBR Green I. b Detection of LAMP products by agarose gel electrophoresis. Lane M: DNA 2 K plus Marker; tubes and lanes 1–5: primer set Tub-E198K; tubes and lanes 6–10: primer set Tub-E198A; tubes and lanes 11–15: primer set Tub-E198V; tubes and lanes 1, 6, 11: ddH2O; tubes and lanes 2, 7, 12: HBStr-104; tubes and lanes 3, 8, 13: HBStr-155; tubes and lanes 4, 9, 14: HBStr-114; tubes and lanes 5, 10, 15: HBStr-496

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