Fig. 3
Sensitivity comparison of LAMP and conventional PCR for the detection of the E198V mutation using plasmid pMD18-TubA. a LAMP products detected with 1000 × SYBR Green I. b LAMP assessment based on agarose gel electrophoresis. c Conventional PCR assessment based on agarose gel electrophoresis. Lane M: DNA 2 K plus Marker; tubes and lanes 1–9: concentration of plasmid pMD18-TubA at 2 × 108, 2 × 107, 2 × 106, 2 × 105, 2 × 104, 2 × 103, 2 × 102, 2 × 101, and 2 × 100 copies/μL, respectively; tube and lane 10: ddH2O