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Table 1 Design of LAMP primers for detection of the E198K/A/V mutations of TUB2 in Botrytis cinerea

From: Rapid detection of benzimidazole resistance in Botrytis cinerea by loop-mediated isothermal amplification

Primer

Type

Length (bp)

Sequence (5′-3′)

F3

Forward outer

21

GCAACTCTCTCTGTCCATCAA

B3

Backward outer

21

CCAACTTTCGGAGATCTGAGT

BIP

Backward inner(B1c-B2)

42

TCTTAACCACTTGGTTTCCGCCGAAGTTGACCAGGGAAACGG

Tub-E198V

Forward inner(F1c-F2)

47

GGTTGCTGAGCTTCAAGGTTCTCACAATTGGTTGAGAACTCTGAGbGTa

Tub-E198A

Forward inner(F1c-F2)

47

GGTTGCTGAGCTTCAAGGTTCTCACAATTGGTTGAGAACTCTGATbGCa

Tub-E198K

Forward inner(F1c-F2)

46

GGTTGCTGAGCTTCAAGGTTCTCACAATTGGTTGAGAACTCTGTbCAa

  1. aNecleotides in bold are modified from the sequence of the TUB2 gene in the sensitive and the resistant isolates
  2. bNecleotides underlined are mismatches introduced specifically to distinguish B.cinerea genotypes (E198K/A/V)