Primer
|
Type
|
Length (bp)
|
Sequence (5′-3′)
|
---|
F3
|
Forward outer
|
21
|
GCAACTCTCTCTGTCCATCAA
|
B3
|
Backward outer
|
21
|
CCAACTTTCGGAGATCTGAGT
|
BIP
|
Backward inner(B1c-B2)
|
42
|
TCTTAACCACTTGGTTTCCGCCGAAGTTGACCAGGGAAACGG
|
Tub-E198V
|
Forward inner(F1c-F2)
|
47
|
GGTTGCTGAGCTTCAAGGTTCTCACAATTGGTTGAGAACTCTGAGbGTa
|
Tub-E198A
|
Forward inner(F1c-F2)
|
47
|
GGTTGCTGAGCTTCAAGGTTCTCACAATTGGTTGAGAACTCTGATbGCa
|
Tub-E198K
|
Forward inner(F1c-F2)
|
46
|
GGTTGCTGAGCTTCAAGGTTCTCACAATTGGTTGAGAACTCTGTbCAa
|
- aNecleotides in bold are modified from the sequence of the TUB2 gene in the sensitive and the resistant isolates
- bNecleotides underlined are mismatches introduced specifically to distinguish B.cinerea genotypes (E198K/A/V)