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Fig. 1 | Phytopathology Research

Fig. 1

From: Engineer complete resistance to Cotton Leaf Curl Multan virus by the CRISPR/Cas9 system in Nicotiana benthamiana

Fig. 1

Schematic diagrams of constructions of CLCuMuV and CLCuMuB infectious clones and CRISPR/Cas9 system-mediated virus resistance in plants. a Genome organization of the wild-type CLCuMuV and CLCuMuB and sgRNA target sites. The ORFs are represented by filled arrows, CR, SCR and A-Rich region are represented by filled boxes. The two target sites are represented by dashed arrowheads. b Schematic representation of CRISPR/Cas9 system-mediated CLCuMuV resistance. Once the virus infects a plant cell, it enters the cell nucleus where it replicates to produce more viruses. Some of them are packaged into virus, which can be transmitted into other plants by whitefly

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