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Fig. 3 | Phytopathology Research

Fig. 3

From: Engineer complete resistance to Cotton Leaf Curl Multan virus by the CRISPR/Cas9 system in Nicotiana benthamiana

Fig. 3

CRISPR/Cas9-mediated targeted cleavage of the CLCuMuV genome. a GFP signals from N. benthamiana plant leaves. No fluorescence was observed in leaves infiltrated only with A. tumefaciens lines containing Cas9 and fsGFP respectively. Cas9 + fsGFP+gRNA-1 is the representative confocal image of leaf cells infiltrated with a mixture of two Agrobacterium cultures — one with a vector containing a fsGFP gene and the other with a vector containing the Cas9 gene and gRNA-1. Cas9 + fsGFP+gRNA-2 is the representative confocal image of leaf cells infiltrated with a mixture of two A. tumefaciens cultures — one with a vector containing a fsGFP gene and the other containing the Cas9 gene and gRNA-2. Leaves were photographed at 48 h after A. tumefaciens infiltration. Bars = 20 μm. b Partial DNA sequences of two fsGFP genes and repaired outcomes following cleavage by Cas9/gRNA complex, and the representative Sanger sequencing chromatographs. Start codons are shown in light blue, gRNA target sequences are shown in red

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