Fig. 4

Symptoms on N. benthamiana plants inoculated with the infectious CLCuMuV and CLCuMuB clones. a Representative images of control and transgenic plants 35 days (dpi) with CLCuMuV and CLCuMuB. 1 to 4 represent Cas9 + gRNA-1 + gRNA-2 transgenic line1, Cas9 + gRNA-1 + gRNA-2 transgenic line8, Cas9 transgenic, and WT, respectively. Bar = 8 cm. b The phenotype of the third, fourth and fifth leaves at the top of transgenic plants 20 dpi with CLCuMuV and CLCuMuB. Group 1 and 2 represent Cas9 + gRNA-1 + gRNA-2 transgenic line1 and Cas9 transgenic, respectively. Bar = 3 cm