Fig. 5

Testing of CLCuMuV genomic DNA accumulation. Southern blot analysis of CLCuMuV genomic DNA accumulation in control and transgenic plants 35 dpi with CLCuMuV. CLCuMuV genomic DNA was detected with DIG-labeled random primed probes against the sequence of CLCuMuV. 1 to 4 represent WT, Cas9 transgenic, Cas9 + gRNA-1 + gRNA-2 transgenic line1, and Cas9 + gRNA-1 + gRNA-2 transgenic line8, respectively