Fig. 5From: Engineer complete resistance to Cotton Leaf Curl Multan virus by the CRISPR/Cas9 system in Nicotiana benthamianaTesting of CLCuMuV genomic DNA accumulation. Southern blot analysis of CLCuMuV genomic DNA accumulation in control and transgenic plants 35 dpi with CLCuMuV. CLCuMuV genomic DNA was detected with DIG-labeled random primed probes against the sequence of CLCuMuV. 1 to 4 represent WT, Cas9 transgenic, Cas9 + gRNA-1 + gRNA-2 transgenic line1, and Cas9 + gRNA-1 + gRNA-2 transgenic line8, respectivelyBack to article page