Fig. 6

The effect of GPCR inhibitor treatment on the symptom of clubroot. Clubroot symptoms (a, b, c) and quantification of Plasmodiophora brassicae DNA (d) in infected roots of the host plants treated or not with GPCR inhibitor. a Illustration of clubroot symptoms of GPCR inhibitor non-treated and treated plants at 28 dpi. Bar = 1 mm. b The transverse section of plant root tissues were observed by SEM. The red arrows indicate the typical infection structure of pathogen. Bar = 10 μm. c Clubroot symptoms of 70 infected roots from three biological repeats were evaluated by using percentage of plants in individual disease classes and DI at 21 and 28 dpi. Asterisks indicate statistically significant differences at the level of P < 0.01 according to Student’s t test. d At 28 dpi, the infected roots of mock-treated plants and inhibitor-treated plants were divided into two groups (A: severe; B: less severe). The relative accumulated amount of pathogen DNA was quantified by qPCR. Error bars represent the SE with three technical repeats. e The expression levels of the primary downstream genes of GPCR in cancer related pathway. Error bars represent the SD with three technical repeats