Fig. 3
UV_1261 suppresses plant basal defense responses and promotes powdery mildew infection in Arabidopsis. a Flg22-induced ROS burst in Arabidopsis leaves stably expressing UV_1261-eYFP or wild-type Col-gl leaves (CK). b Comparison of flg22-induced callose deposition in leaves of UV_1261-eYFP transgenic plants and Col-gl. c Expression profiling of indicated defense-related genes in UV_1261-eYFP-expressing Arabidopsis and Col-gl treated with flg22. AtACT2 was used as a reference gene. Relative expression was normalized to that in Col-gl at 0 h, and calculated by the comparative CT method 2–ΔΔCT. N. D., not detected. Error bars indicate standard deviations (n = 3). d Disease symptom of Col-gl and UV_1261-eYFP-expressing Arabidopsis inoculated with powdery mildew isolate Golovinomyces cichoracearum SICAU1. White fungal mass was observed on leaf surface (indicated by white arrows). e Quantification of powdery mildew spores on leaves of indicated plants. Significant difference was determined by the Student’s t-test. **, P < 0.01. f Trypan blue staining of infected leaves from Col-gl and UV_1261-eYFP-expressing Arabidopsis. Much more mycelia were seen on UV_1261-expressing leaves than on Col-gl leaves