Fig. 5

Mutants of the de novo pathway of phospholipid biosynthesis exhibited attenuated virulence on wheat heads. a Infected wheat heads were examined 15 days after inoculation with a conidial suspension of each strain. The inoculation sites are indicated by the black dots. b Comparison of DON production between the wild-type strain and mutants after 7 days of incubation in the DON-inducing medium TBI. c Growth inhibition of the wild-type strain and mutants in response to H₂O₂. Strains were grown on CM amended with or without 0.05% H₂O₂. Images were obtained when the mycelia of the wild-type strain reached the edge of the plate. d Percentage of mycelial growth inhibition by 0.05% H₂O₂ in each strain. The vertical lines on the bars in each column denote the standard deviations of three repeated experiments. The values corresponding to the bars denoted with the same letter are not significantly different according to the (LSD) test at P = 0.01