Skip to main content

Table 2 Fitness components, control efficacy and stability of Botrytis cinerea isolates from strawberry

From: Fungicide resistance of Botrytis cinerea from strawberry to procymidone and zoxamide in Hubei, China

Phenotype Isolate Resistance levelsa Mycelium growth b Osmotic sensitivity 8% (PIMG) c Virulence (cm) d Sporulation (×  106/cm2)e Control efficacy f Resistance stability EC50 (μg/mL)g
1st 10th
ProS S191 S 6.57 90.84 2.20 4.8 100
ProS S377 S 6.57 97.85 2.23 4.0 100
ProS S524 S 6.28 95.23 2.60 2.5 100
ProS S572 S 6.26 95.21 2.45 2.1 100
  Mean   6.42A 94.78B 2.37A 3.36A 100A
ProR S106 LR 5.61 104.66 2.62 6.2 23.37 1.52 2.176
ProR S111 LR 5.98 101.61 2.39 3.2 11.56 2.45 2.799
ProR WH36 LR 6.68 107.85 2.23 5.4 16.28 1.56 2.397
ProR YD4 LR 4.59 110.49 2.35 3.2 24.36 1.64 3.338
  Mean   5.72A 106.15A 2.4A 4.52A 18.89B 1.79A 2.67A
ProR S151 MR 6.46 102.29 2.33 4.3 12.52 3.48 4.511
ProR S155 MR 6.68 103.02 2.54 6.3 21.05 4.05 5.999
ProR S299 MR 6.26 106.78 2.40 2.2 23.13 3.86 2.779
ProR YD3 MR 7.16 103.08 2.61 7.6 18.95 3.61 6.437
  Mean   6.64A 103.79A 2.47A 5.11A 18.91B 3.75A 4.93A
ZoxS S104 S 7.12A 2.98A 3.83B 100A
ZoxR S295 R 6.43 2.91 6.4 7.12 4.32 4.72
ZoxR S329 R 6.74 2.01 7.2 20.29 4.53 4.41
ZoxR S196 R 6.30 2.38 7.0 13.98 20.25 19.22
ZoxR S133 R 7.08 3.54 6.8 11.01 4.83 4.59
ZoxR S155 R 7.16 2.84 5.0 22.45 6.73 6.87
ZoxR S298 R 7.33 2.1 5.4 18.22 5.95 6.11
  Mean   6.84A 2.64A 6.38A 15.51B 7.76A 7.65A
  1. a Resistance levels of procymidone resistant isolates. LR and MR represent low and moderate resistance, respectively; b Mycelium growth was measured as colony diameter (cm) at 60 h under 23 °C; c The percentage of inhibition of mycelial growth (PIMG) was calculated using the formula PIMG = [(C-N)/(C-5)] × 100, where C is the colony diameter of the non-treatment control, and N is that of the sodium chloride treatment; d Virulence was shown as the diameter of lesion size (cm) at 3 days under 23 °C; e Mean number (×106) of conidia per square centimeter; f Control efficacy was expressed as the percentage of the mean diameter of the control minus the mean diameter of the treatment divided by the mean diameter of the control; g Stability of procymidone/zoxamide resistant isolates after 10 consecutive transfers on fungicide free medium (1st and 10th transfer), means were compared between 1st and 10th transfers of each group. Means indicate the sensitive and corresponding resistant groups, values within a column followed by the same letter are not significantly different according to LSD test (P = 0.05)