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Table 2 Fitness components, control efficacy and stability of Botrytis cinerea isolates from strawberry

From: Fungicide resistance of Botrytis cinerea from strawberry to procymidone and zoxamide in Hubei, China

Phenotype

Isolate

Resistance levelsa

Mycelium growth b

Osmotic sensitivity 8% (PIMG) c

Virulence (cm) d

Sporulation (×  106/cm2)e

Control efficacy f

Resistance stability EC50 (μg/mL)g

1st

10th

ProS

S191

S

6.57

90.84

2.20

4.8

100

–

–

ProS

S377

S

6.57

97.85

2.23

4.0

100

–

–

ProS

S524

S

6.28

95.23

2.60

2.5

100

–

–

ProS

S572

S

6.26

95.21

2.45

2.1

100

–

–

 

Mean

 

6.42A

94.78B

2.37A

3.36A

100A

–

–

ProR

S106

LR

5.61

104.66

2.62

6.2

23.37

1.52

2.176

ProR

S111

LR

5.98

101.61

2.39

3.2

11.56

2.45

2.799

ProR

WH36

LR

6.68

107.85

2.23

5.4

16.28

1.56

2.397

ProR

YD4

LR

4.59

110.49

2.35

3.2

24.36

1.64

3.338

 

Mean

 

5.72A

106.15A

2.4A

4.52A

18.89B

1.79A

2.67A

ProR

S151

MR

6.46

102.29

2.33

4.3

12.52

3.48

4.511

ProR

S155

MR

6.68

103.02

2.54

6.3

21.05

4.05

5.999

ProR

S299

MR

6.26

106.78

2.40

2.2

23.13

3.86

2.779

ProR

YD3

MR

7.16

103.08

2.61

7.6

18.95

3.61

6.437

 

Mean

 

6.64A

103.79A

2.47A

5.11A

18.91B

3.75A

4.93A

ZoxS

S104

S

7.12A

–

2.98A

3.83B

100A

–

–

ZoxR

S295

R

6.43

–

2.91

6.4

7.12

4.32

4.72

ZoxR

S329

R

6.74

–

2.01

7.2

20.29

4.53

4.41

ZoxR

S196

R

6.30

–

2.38

7.0

13.98

20.25

19.22

ZoxR

S133

R

7.08

–

3.54

6.8

11.01

4.83

4.59

ZoxR

S155

R

7.16

–

2.84

5.0

22.45

6.73

6.87

ZoxR

S298

R

7.33

–

2.1

5.4

18.22

5.95

6.11

 

Mean

 

6.84A

–

2.64A

6.38A

15.51B

7.76A

7.65A

  1. a Resistance levels of procymidone resistant isolates. LR and MR represent low and moderate resistance, respectively; b Mycelium growth was measured as colony diameter (cm) at 60 h under 23 °C; c The percentage of inhibition of mycelial growth (PIMG) was calculated using the formula PIMG = [(C-N)/(C-5)] × 100, where C is the colony diameter of the non-treatment control, and N is that of the sodium chloride treatment; d Virulence was shown as the diameter of lesion size (cm) at 3 days under 23 °C; e Mean number (×106) of conidia per square centimeter; f Control efficacy was expressed as the percentage of the mean diameter of the control minus the mean diameter of the treatment divided by the mean diameter of the control; g Stability of procymidone/zoxamide resistant isolates after 10 consecutive transfers on fungicide free medium (1st and 10th transfer), means were compared between 1st and 10th transfers of each group. Means indicate the sensitive and corresponding resistant groups, values within a column followed by the same letter are not significantly different according to LSD test (P = 0.05)