Table 3 Primers used in this study to amplify BcOs1 and BctubA gene fragments
From: Fungicide resistance of Botrytis cinerea from strawberry to procymidone and zoxamide in Hubei, China
| Primer | Sequence (5′-3′) | Location in gene | Reference |
|---|---|---|---|
|
Bc-f (forward) Bc-r (reverse) |
CAGGAAACACTTTTGGGGATA GAGGGACAAGAAAATCGACTAA | Amplify 327 bp BcOs1 gene fragment of B. cinerea | (Fan et al. 2015) |
|
BF1 (forward) BR1 (reverse) |
TACCGATCGAAAAACCCAAC TGGGCTGGTCTCTCAATCTT | Amplify 990 bp (95–1085) BcOs1 gene fragment of B. cinerea | (Ma et al. 2007) |
|
BF2 (forward) BR2 (reverse) |
CAACGTTATGGCACAAAATCTCA AAGTTTCTGGCCATGGTGTTCA | Amplify 848 bp (981–1829) BcOs1 gene fragment of B. cinerea | |
|
P1 (forward) P2 (reverse) |
ATGCGTGAGATTGTATGTATTTC CTATTCCTCGCCCTCAATTG | Amplify 1765 bp β-tubulin gene fragment of B. cinerea | (Liu et al. 2010) |
|
Bc365PM (forward) Bc365PM (reverse) |
GAAAGCGAAGGCGTCCAG GTCTCCCTTTGCGACAGC |
Allele specific PCR primers to detect I365S point mutation in BcOs1 gene | This study |