Fig. 2

Pns11 specifically interacted with gelsolin in cultured cells. a The western blot analysis showing the specificity of gelsolin antibodies. Samples were separated by SDS-PAGE and detected with gelsolin-specific antibodies. Lane M, protein ladder; lanes 1 and 2, protein extracts from cultured cells and insect bodies; lane 3, negative control, i.e., PBS. b Gelsolin expressing as the thread-like structures in mock-infected cultured cells, and Pns11 expressing in tubule structures protruding from the cell surface in RGDV-infected cells. c Gelsolin co-localizing with Pns11 tubules in RGDV-infected cultured cells. At 44 hpi, cells were immunolabeled for gelsolin with gelsolin-rhodamine (red) and for Pns11 tubules with Pns11-FITC (green), then examined by confocal microscopy. White arrows indicate the co-localization of Pns11 and gelsolin. Bars, 10 μm