Fig. 2

Northern hybridization analyses of P. sojae Avr1b and Avr1k sRNAs during soybean infection. a The accumulation of Avr1b-siRNAs is associated with Avr1b⁻ virulence phenotype. The black triangle indicates Avr1b-siRNA. b The accumulation of Avr1b-asRNA is associated with Avr1b⁻ virulence phenotype. Except for Avr1b-asRNA (indicated by the white triangle), a constitutive host sRNA (Gma_1b-asRNA, indicated by the gray triangle) was also detected. c Northern blot failed to detect Avr1b-asRNA antisense. d Avr1k-sRNA accumulation is associated with its virulence phenotype. e Northern blot of U6 which served as a loading control. The total RNAs of 0–84 h infected tissues of G. max cultivar Williams (rps1b and rsps1k) were used. For the sample of 0 h, no P. sojae mycelia were applied. “Avr” and “avr” in the upper panel indicate avirulent or virulent genes, respectively. Each lane was loaded with 20 μg total RNA