Fig. 2

Hybridization buffer prevented complete denaturation of cDNAs. The driver with hybridization buffer (a) or 10 mM Tris-HCl (b) were denatured at 98 °C for 2 min, and then treated with DSN at 68 °C for 10 min and subsequently inactivated at 98 °C for 5 min. Using denatured driver cDNAs as template, genes OsActin and OsCAT-A were amplified by gene-specific primers. Aliquots of PCR reaction mixture were taken at 20, 25, 30, and 35 cycles of amplification and the products were analyzed on 1% agarose gels as indicated. “DSN1” and “DSN2” represent repeated treatment with DSN, and “Non-DSN” indicates samples without DSN treatment in the reaction mixture. Lane M, DNA ladder (Generay. Code No. 1705G22)