Subtractive hybridization-assisted screening and characterization of genes involved in the rice-Magnaporthe oryzae interaction

Chang, Qing-Le; Xu, Hai-Jiao; Peng, You-Liang; Fan, Jun

doi:10.1186/s42483-019-0027-5

Table 2 Information of the candidate gene-encoding proteins

From: Subtractive hybridization-assisted screening and characterization of genes involved in the rice-Magnaporthe oryzae interaction

Clone No.	Gene	Encoding protein	Cell death^a	Induced chlorosis^b	Source
2G7, 38F1, 38G4, 65D9	LOC4324770	Myb-related protein 308	+++	Yes^c	Rice
41E7, 47H4, 70G11	LOC4346246	Myb-related protein Zm38	++	Yes	Rice
58F2	LOC4330027	Myb4-like protein	+	Yes	Rice
32F1, 33G9	LOC9266213	Transcription factor Myb 8	++	No	Rice
16C1, 16E6, 16F5, 23B10, 24G5, 25A9, 32A11, 32G5, 35A4, 49A2	LOC4333423	Zinc finger protein 1	+++	Yes	Rice
19D7, 30A11, 36G8, 38E2, 52G1, 55A12, 65H11	LOC9267830	Zinc finger protein 2	++	Yes	Rice
30A8	LOC4330898	Zinc finger protein 8	++	Yes^c	Rice
31A8	LOC4346072	RanBP2 zinc finger protein	++	No	Rice
47G1	LOC4335388	OsWRKY17	+	No	Rice
3A2	LOC4341678	WRKY28-like protein	+++	No	Rice
11B7	LOC4338413	OsWRKY70	+	No	Rice
60G5	LOC107278717	OsWRKY72	++	Yes	Rice
35E11	LOC4349267	Chitinase 8	+++	Yes^c	Rice
6H5	LOC4331519	Chitinase 11	++	Yes^c	Rice
12D8	LOC4346014	AT-hook motif nuclear-localized protein 10	++	No	Rice
14E3	LOC4331447	33 kDa ribonucleo protein, chloroplastic	++	No	Rice
23B9	LOC4333394	Peptidyl-prolyl cis-trans isomerase E	+	Yes^c	Rice
45F10	LOC4332476	Serine/arginine-rich splicing factor RS2Z32	+++	Yes	Rice
45G11	LOC4331546	Pre-mRNA splicing factor cwf23	+++	No	Rice
52F8	LOC4328486	CASP-like protein 2C2	+	No	Rice
53H9	LOC4332413	Heat shock cognate 70 kDa protein 2	++	Yes	Rice
4G7	LOC4326977	Ubiquitin-40S ribosomal protein S27a-1	+++	No	Rice
13F5	LOC4329736	Protein sensitive to proton rhizotoxicity 1	++	No	Rice
5A6, 11A10, 12C4, 65B12	LOC4342269	Uncharacterized protein	+++	No	Rice
14B9, 14F1	LOC4334216	Uncharacterized protein	+++	Yes	Rice
16D8	LOC9271812	Uncharacterized protein	++	Yes	Rice
23B5	LOC4324656	Uncharacterized protein	+	No	Rice
42G4, 52F10	LOC9267428	Uncharacterized protein	++	No	Rice
44A3	LOC4332848	Uncharacterized protein	+	Yes	Rice
44F6	LOC9268105	Uncharacterized protein	+	No	Rice
5A10	MGG_08356	Polysaccharide deacetylase	++	No	Blast fungus
4H10, 7F10	MGG_10522	Hypothetical protein	++	Yes	Blast fungus

^a Cell death was observed on N. benthamiana; ^b Chlorosis on barley leaves was induced by gene expression with or without subsequent infection by the blast fungus; ^c Chlorosis could be induced in absence of the fungal infection; “+++” indicates tissue collapse of the Agrobacteria-inoculated region; “++” and “+” indicate partial or sporadic cell death, respectively, observed on the lower surface of leaves

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