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Fig. 3 | Phytopathology Research

Fig. 3

From: Ralstonia solanacearum virulence in eggplant seedlings by the leaf-clip inoculation

Fig. 3

Fluorescence microscopy of eggplant seedlings after 4 dpi by the leaf-clip method inoculated with a Water, b mCherry tagged R. solanacearum (TRS1016), c hrpB mutant (TRS1017) and d phcA mutant (TRS1018). F1C1 hrpB mutant was tagged with mCherry (TRS1017) and the phcA mutant was tagged with mCherry (TRS1018). TRS1016 (F1C1 wild type tagged with mCherry), TRS1017 and TRS1018 inoculated eggplant seedlings were observed under the fluorescence microscope at 4 dpi. In the case of TRS1016, fluorescence was observed in leaf, stem as well as in root regions, whereas in the case of TRS1017 fluorescence was largely limited to the region in the leaf where inoculation was done and was very faint in stem and root regions, which indicated that the hrpB mutant is growth deficient inside the seedling. In the case of TRS1018, fluorescence was observed in leaf, stem and root like TRS1016, which indicated that phcA mutant is able to grow and spread better than hrpB mutant in eggplant seedlings

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