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Fig. 4 | Phytopathology Research

Fig. 4

From: Ralstonia solanacearum virulence in eggplant seedlings by the leaf-clip inoculation

Fig. 4

Virulence of R. solanacearum F1C1 wild type, hrpB, hrpG and phcA mutants in eggplant seedlings tested by the leaf-clip inoculation method. Eggplant seedlings were inoculated by the F1C1 wild type, hrpB, hrpG or phcA mutants with saturated concentrations (109 CFU/mL). X-axis represents dpi and Y-axis represents the percentage of seedlings killed. On 6 dpi, 100% of seedlings were killed upon inoculation with F1C1 wild type, in the case of the phcA, till 4 dpi, almost all the seedlings were healthy, but by 9 dpi, more than 80% seedlings were killed. For hrpB mutant, by 10 dpi only 3% seedlings exhibited disease symptoms. We observed 5% seedlings death by 10 dpi for hrpG mutant. This suggested that phcA mutant is virulence deficient compared to wild type (P < 0.001; log-rank test) while hrpB and hrpG mutants are almost non-pathogenic. All data point in the line graph is an average of three independent experiments with two replicates. Error bars are depicting standard errors

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