Fig. 5

A representative picture showing differential pathogenicity of R. solanacearum between eggplant and tomato seedlings by recruiting their seedlings in a single microfuge tube. Both eggplant (marked with red star) and tomato seedlings (marked with red circle) were kept in one microfuge tube and then inoculated with a water b F1C1 wild type c phcA mutant d hrpB mutant and e hrpG mutant. Observations were made on each day to score disease in eggplant and tomato seedlings. The picture was taken at 4 dpi. It was distinct that eggplant seedlings were killed earlier than the tomato seedlings in the tube inoculated with F1C1 wild type strain. For inoculation with phcA mutant, eggplant seedlings developed disease while tomato seedlings were healthy in the same tube. For hrpB and hrpG mutants, both eggplant and tomato seedlings were healthy like the water inoculated seedlings. For hrpG, few seedlings in both hosts died towards the later dpi. The differential aggression of R. solanacearum in tomato and eggplant seedlings by leaf-clip inoculation was very distinct