Table 1 Variation of clones with nucleotide deletion/mutation in sequence of the short non-coding sequence, UPD and TLS in TMV(24A+UPD) during serial passaging
Serial passaging | Number of clones with nucleotide deletion/mutation |
|---|---|
1st | 5 (1 nt: 4; 2 nt: 1) |
2nd | 7 (1 nt: 6; 2 nt: 1) |
3rd | 8 (1 nt: 7; 2 nt: 1) |
4th | 13 (1 nt: 8; 2 nt: 5) |
5th | 28 (1 nt: 12; 2 nt: 13; 3 nt: 2; 4 nt: 1) |
6th | 32 (2 nt: 28; 3 nt: 2; 4 nt: 1; 10 nt: 1) |
7th | 32 (1 nt: 2; 2 nt: 20; 3 nt: 8; 4 nt: 2) |
8th | 32 (2 nt: 20; 3 nt: 5; 22 nt: 2; 25 nt: 3; 42 nt: 2) |
9th | 32 (2 nt: 32) |
10th | 32 (23 nt: 32) |