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Fig. 2 | Phytopathology Research

Fig. 2

From: Spatiotemporal nature of Fusarium graminearum-wheat coleoptile interactions

Fig. 2

Laser confocal microscopic images of F. graminearum infection of wheat coleoptiles at 8 hpi. Tissue cells of wheat coleoptiles were stained with PI (Propidium iodide) (red). a, b A conidium of F. graminearum germinated into epiphytic runner hyphae on the extracellular surface of wheat coleoptiles. After hyphal branching, multiple appressoria-like structures are formed at the hyphal tip. Arrowheads indicate appressoria-like structures. Detailed views of each boxed region are shown at the bottom of the respective images. These images are 3D (three-dimensional) reconstructions of 65 optical sections acquired with a z-interval of 0.4 μm. c A 3D image showing a hypha growing in an intercellular space of a wheat coleoptile cell. Clear and intact cellular outlines were stained by PI, indicating that wheat coleoptile cells were alive at this stage. This image is a 3D reconstruction of 201 optical sections acquired with a z-interval of 0.15 μm. d A proposed model depicting the early stages (5–8 hpi) of F. graminearum proliferation inside wheat coleoptiles

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