Fig. 5

F. graminearum uses biotrophic invasion strategy to infect wheat coleoptile cells. Staining patterns with saturated FM4–64 in wheat coleoptile cells invaded by GFP-labeled fungal strain PH-1-GFP (green). Arrows indicate FM4–64–labeled membranes outside the invasive hyphae (IH). Arrowheads indicate FM4–64–labeled vacuolar membranes of the wheat coleoptile cells. Intracellular IH are sealed within the host membrane either in F. garaminearum invasive growth and colonization (a) or cell-to-cell invasion (b). a This image is a 3D reconstruction of 7 optical sections acquired with a z-interval of 1 μm. b This image is a 3D reconstruction of 19 optical sections acquired with a z-interval of 1 μm. DIC, differential interference contrast. GFP, green fluorescent protein