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Fig. 6 | Phytopathology Research

Fig. 6

From: Spatiotemporal nature of Fusarium graminearum-wheat coleoptile interactions

Fig. 6

Spatial and temporal development of IH, and plant responses by callose deposition in the invaded wheat coleoptiles cells. Coleoptiles were stained with aniline blue and callose deposition was pictured at the indicated time-points. Detailed views of each boxed region are shown at the bottom of the respective images. a The mock-inoculated control coleoptile cells were poorly labeled with aniline blue, therefore showing limited discrete foci at the cell wall. b Callose signals formed continuous lines along the hyphal fronts before the swelling hyphae penetrate the wheat coleoptile cell wall (8 hpi) by means of a peg (arrowhead). This image is a 3D reconstruction of 101 optical sections acquired with a z-interval of 0.4 μm. c Callose accumulated dramatically when a hypha begins to traverse the host cell wall (16 hpi). Arrowheads indicate penetration sites. Asterisks indicate the pores due to peg penetration (without callose deposition). This image is a 3D reconstruction of 101 optical sections acquired with a z-interval of 0.3 μm. d Extensive fungal growth as seen in infected wheat coleoptiles cells at 24 hpi. Callose was not only deposited between the neighboring cell walls but also decorated at the outer linings of the invasive hyphae. This image is a 3D reconstruction of 128 optical sections acquired with a z-interval of 0.28 μm

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