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Fig. 2 | Phytopathology Research

Fig. 2

From: A putative N-BAR-domain protein is crucially required for the development of hyphae tip appressorium-like structure and its plant infection in Magnaporthe oryzae

Fig. 2

Subcellular localization of MoBar-A and MoBar-B in Magnaporthe oryzae and the expression level of their respective genes during fungal infection. a The localization pattern of GFP/MoBar-A and MoBar-B/GFP in asexual spore and during spore germination and appressorium formation. Localization of GFP/MoBar-A and MoBar-B/GFP were visualized by Nikon laser confocal and laser excitation epifluorescence microscopy, the scale bar is 10 μm. b The relative expression level of MoBAR-A and MoBAR-B in the asexual spores and at different stages of infection in-planta by qPCR. The error bars represent standard errors from at least three independent replicates (*, P < 0.05 by t-test). The expression of M. oryzae actin gene in the mutants and the wild-type strain at each stage of infection was used as the internal control. The expression level of MoBAR-A and MoBAR-B during the development of vegetative hyphae was used as control stage and was assumed as unity (the expression level of MoBAR-A and MoBAR-B at hyphal stage =1)

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