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Fig. 4 | Phytopathology Research

Fig. 4

From: A novel gene from a secondary metabolism gene cluster is required for microsclerotia formation and virulence in Verticillium dahliae

Fig. 4

Silencing of Nag1 causes delayed growth and reduced conidial production and germination. a Colony morphology of the wild-type strain (WT) and two Nag1-silenced mutants. The pictures were taken at 7 dpi on PDA plates at room temperature. b Colony diameter statistics of WT, Nag1-S3, and Nag1-S6 at 7 dpi on PDA plates at room temperature. Error bars were derived from three independent experiments. c Number of conidia produced by WT, Nag1-S3, and Nag1-S6 at 8 dpi on PDA plates at room temperature. Error bars were derived from three independent experiments. d The percentage of conidial germination after 2 days of growth in liquid BM at 150 rpm at 25 °C. Error bars indicate standard deviation from three independent experiments. Different letters in (b), (c), (d) indicate significant differences at P ≤ 0.05

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