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Fig. 7 | Phytopathology Research

Fig. 7

From: A novel gene from a secondary metabolism gene cluster is required for microsclerotia formation and virulence in Verticillium dahliae

Fig. 7

Silencing of Nag1 leads to reduced melanin biosynthesis. a Accumulation of melanin in WT, Nag1-S3, and Nag1-S6 7 at 14 dpi in Czapek salt medium (150 rpm, 25 °C). b Relative expression levels of genes related to melanin synthesis. Total RNA was isolated from cultures after 7 days of growth in Czapek salt medium (150 rpm at 25 °C). Relative expression levels were determined by qRT-PCR using the ΔΔCT method, with β-tubulin as a reference. Error bars indicate standard deviation from three independent experiments

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