Fig. 4
Effect of 4-HBA on the CdgL-LysR binding and the CdgL-LysR-DNA ternary complex formation. a Microscale thermophoresis (MST) showing that GST-CdgL interacted with LysR-His6 with a Kd of 0.56 μM. FNorm was plotted on a linear y-axis in per mil (‰) against the total concentration of thetitrated partner on a log10 x-axis as described previously (Seidel et al. 2013). b, c, d Characterization of the LysR-CdgL binding affinity via MST in the presence of various concentrations of 4-HBA: 0.5 μM (b),1 μM (c), or 5 μM (d). e Effect of 3-HBA on the interaction between CdgL and LysR. f Effect of 4-HBA on the LysR-CdgL-DNA ternary complex formation determind by electrophoretic mobility shift assay