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Fig. 1 | Phytopathology Research

Fig. 1

From: A non-flagellated biocontrol bacterium employs a PilZ-PilB complex to provoke twitching motility associated with its predation behavior

Fig. 1

PilZLe3639 is a stand-alone PilZ domain and unable to bind c-di-GMP. a Multiple sequence alignment of the PilZLe3639-like proteins. PilZLe3639, PilZPA2960, Lca55_3236, Lgu3211_1674, Lde18482_475, Lar79_2362, Lco07_960, PilZXac1133 and PilZXc1028 indicated these stand-alone PilZ domains from Lysobacter enzymogenes OH11, Pseudomonas aeruginosa PA01, L. capsisi 55, L. gumosus 3.2.11, L. defluvii DSM18482, L. arseniciresistens ZS79, L. concretionis Ko07, Xanthomonas citri and X. campestris, respectively. YcgR (PDB entry 5Y6G), is a c-di-GMP-binding protein from E. coli. The RxxxR and [D/N] xSxxG that are required for c-di-GMP binding (Guzzo et al. 2009) are highlighted by red boxes. Two conserved residues (Y24 and W71) in the selected stand-alone PilZ domains are indicated by purple and green triangles, respectively. b Microscale thermophoresis (MST) assay showed PilZLe3639 failed to bind with c-di-GMP, while c Clp, a known c-di-GMP-binding protein (Xu et al. 2018), showed moderate binding affinities with c-di-GMP determined by MST

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Phytopathology Research

ISSN: 2524-4167