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Fig. 2 | Phytopathology Research

Fig. 2

From: A non-flagellated biocontrol bacterium employs a PilZ-PilB complex to provoke twitching motility associated with its predation behavior

Fig. 2

PilZLe3639 and its residue W71 both played essential roles in the formation of twitching motility by L. enzymogenes. Mutation of pilZLe3639 in wild-type OH11 led to a complete defect in forming twitching motility, as no twitching cells (indicated by blight arrows) were observed at the margin of strain colony. Complementing the wild-type PilZLe3639 or a variant carrying a Y24-A24 substitution (indicated as Y24A) into the mutant both rescued this motile deficiency. Introducing the empty vector (pBBR1-MCS5, abbreviated as pBBR) or the pilZLe3639 variant possessing a W71-A71 replacement (indicated as W71A) failed to rescue the twitching deficiency of the pilZLe3639 mutant

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Phytopathology Research

ISSN: 2524-4167