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Fig. 3 | Phytopathology Research

Fig. 3

From: A non-flagellated biocontrol bacterium employs a PilZ-PilB complex to provoke twitching motility associated with its predation behavior

Fig. 3

PilZLe3639 directly interacted with PilBLe0708, a characterized pilus motor protein.a, b pKT25-PilZLe3639 with the recombinant pUT18c carrying different pilus-associated proteins that are required for twitching motility in L. enzymogenes OH11 (Xia et al. 2018) were co-transformed into the E. coli BTH101 cells to test their potential protein-protein interactions by the bacterial adenylate cyclase two-hybrid (BACTH) system. The co-transformed E. coli colony with a “blue” color indicated the test protein-protein interactions happened under the test conditions. Using this approach, only PilBLe0708 with PilZLe3639 showed an interaction signal. “+”, a positive control (pUT18c-Zip &pKT25-Zip), “-”, a negative control (pUT18c &pKT25). c GST pull-down assay confirmed the direct binding of PilZLe3639 with PilBLe0708 in vitro

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Phytopathology Research

ISSN: 2524-4167