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Fig. 3 | Phytopathology Research

Fig. 3

From: A Xanthomonas oryzae type III effector XopL causes cell death through mediating ferredoxin degradation in Nicotiana benthamiana

Fig. 3

Nb ferredoxin (NbFd) interacts with XopL. a Schematic representation of relevant domains in NbFd and XopL. Chl. P, chloroplast addressing peptide; XopL-LRR, XopL-leucine rich-repeat domain; XL-box, E3 ligase domain containing the XL-box. Numbers above functional maps denote the position of the domains with respect to the start codon. b Yeast two-hybrid assay showing interactions between NbFd and XopL and variants on SD/−Leu-Trp-His-Ade agar medium with and without 20 mg/mL X-α-GAL. Experiments were repeated three times. c CoIP analysis of 3 × flag-XopL and NbFd-YFP in vivo. Leaf extracts were incubated with anti-myc labeled magnetic beads. Samples before (input) and after (IP:α-myc) immunopurification were analyzed by immunoblotting using anti-flag and anti-myc antibodies. White asterisks indicate non-specific reactions with c-myc antisera. Representative picture of three replicates were shown. d BiFC visualization of the interaction between YN-tagged XopL and YC-tagged ferredoxin in Nb leaves. Controls included XopL:YN coinfiltrated with empty YC vector and empty YN vector coinfiltrated with NbFd:YC (NbFd:YC). Chloroplasts were visualized by auto-fluorescence. Bars represent 20 μm. Experiments were performed three times with similar results

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